Crystal violet stain for amyloid

Stain sections of fixed or unfixed tissue in Crystal Violet Solution for three to five minutes. Wash in Acetic Acid, 10%, followed by a thorough washing in water to remove all traces of acid. Examine in water or glycerine. The stain will keep for some time if sections are mounted in a saturated solution of potassium acetate or in levulose Crystal Violet stain for Amyloid . Test number: 12376 - Technical only, 12379 - Technical & interpretation . Excellian order number: LAB12376 LAB12379 . Alternate names: Amyloid Amyloidosis Special stain. STAINING: Stain sections of fixed or unfixed tissue in Crystal Violet Solution (E-301-1) for three to five minutes. Wash in Acetic Acid, 10% (E-301-2), followed by a thorough washing inwater to remove all traces of acid Other methods of detection of amyloid include fluorescent stains, e.g., thioflavin T or S, and metachromatic stains such as crystal violet. Immunofluorescence and immunoperoxidase methods are used to identify and classify amyloid proteins in tissues

Crystal violet, cango red stain are used for demonstrating amyloids In metachromatic stain amyloid appear pink and the surrounding tissuesstain purple Crystal violet stain and congo-red stain are used to demonstrate amyloidin tissues (Figure 5) Crystal violet stain with appropriate controls demonstrated positive staining with amyloid deposits in the papillary dermis. (Figure 6) These pathological findings in this clinical setting were consistent with lichen amyloidosis Amyloid deposition of the breast in primary Sjögren Metachromatic by modified Wright stain (Diagn Cytopathol 2003;28:325 giant cells. Positive stains. Congo red (red orange with apple green birefringence under polarized light) Metachromatic with crystal violet Negative stains. PAS. Electron microscopy description. Straight.

Crystal Violet Stain For Amyloid Technical Data Shee

The crystal violet stain for amyloid is a/an: a) polarization technique b) physical reaction c) impregnation method d) polychromatic stain. d) polychromatic stain. Acid mucopolysachharides are demonstrated by: a) alcian blue b) alizarin red c) Congo red d) crystal violet. a) alcian blue Newcomer Supply Crystal Violet Stain, Lieb, Alcoholic is used to provide rapid screening method for amyloid deposits in tissue sections. This procedure has low sensitivity and should only be considered as an amyloid screening technique and not an amyloid specific stain For amyloid staining, crystal violet is often preferred to methyl violet as the orthochromatic shade is a little bluer and the purple-red of the amyloid contrasts more effectively with this than with the slightly redder methyl violet Other less frequently used stains for cutaneous amyloid include crystal violet, pagoda red and methyl violet.. Cytokeratin (CK) staining can be used to identify the amyloid as epidermal in origin. Positive staining with immunostaining to kappa and lambda light chains is suggestive of AL amyloid deposition and should prompt consideration of systemic amyloidosis Certified for use with the Gram stain for bacteria, Flemming triple stain with iodine for chromosomes, Lieb′s amyloid stain and Newton′s crystal violet-iodine technique for chromatin and nucleoli

Crystal violet staining solution, for Amyloid Supplier: Electron Microscopy Sciences. Synonyms: Hexamethyl Violet, Vianin, Basic Violet 3, Gentian violet, Methyl violet 10B, Hexamethyl-p-rosaniline chloride, CI 42555, 4-[4,4'-Bis(dimethylamino) benzhydrylidene]cyclohexa-2,5-dien-1-ylidene]dimethylammonium chloride, Gentiaverm, Dimethyl((di(4-. Newcomer Supply Crystal Violet Stain, Lieb, Alcoholic is used to provide a rapid screening method for amyloid deposits in tissue sections. This procedure has low sensitivity and should only be considered as an amyloid screening technique and not an amyloid specific stain Methyl or crystal violet metachromasia The triphenylmethane dye, methyl violet, was the first synthetic dye used for the demonstration of amyloid (Cornil 1875). The rationale of the staining reaction remains unexplained

Recent advances in amyloidosis

Crystal Violet stain for Amyloid-12376 - Technical only

  1. Amyloid stains positively with congo red, periodic acid-Schiff (PAS), crystal violet, and toluidine blue. Characteristically, the congo red stain produces an apple-green birefringence under polarised microscopy. Differential diagnosis for systemic amyloidosis. Other conditions that should be considered include
  3. It stains metachromatically with crystal violet and toluidine blue. It stains with congo red and sirius red F3B. Congo red and sirius red stained amyloid show green birefringence. It can be stained with trypan blue

Crystal Violet. Less commonly used to stain amyloid and used to recognize Lattice corneal stromal dystrophy; Purple/violet: amyloid; Thioflavin T. Stains for amyloid and used to recognize Lattice corneal stromal dystrophy in immunocytochemistry; Fluorescent yellow: amyloid; Masson's Trichrome Masson's Trichrome stain in Granular corneal. Amyloid stains pink with hematoxylin and eosin and metachromatically with crystal violet and methyl violet.280 It stains selectively with Congo red; in addition, amyloid stained by Congo red gives an apple-green birefringence when viewed in polarized light I have included below my favoured method for amyloid as is it very quick, only needs light microscopy and produces a very good visual result. Crystal Violet. 1. Sections to water. 2. Stain with crystal violet solution (same as one used in Gram stain) for 2 - 3 mins. 3. Wash in water then diff in very weak (~0.2%) acetic acid for about 5 secs. 4 Most commonly, amyloid can be identified with the Congo red stain as an apple-green birefringence when viewed under polarized microscopic light. Additionally, other stains may be used to identify amyloid such as crystal violet or thioflavin T - Crystal violet: stains amyloid - Apathy mounting medium; prevents bleeding What color is amyloid stained when stained with crystal violet? Purple/rose. What color are the other elements of a specimen when it is stained with crystal violet? Blue/Dark purple

Crystal violet or gentian violet, also known as methyl violet 10B or hexamethyl pararosaniline chloride, is a triarylmethane dye used as a histological stain and in Gram's method of classifying bacteria. Crystal violet has antibacterial, antifungal, and anthelmintic properties and was formerly important as a topical antiseptic Prepared Staining Kits for Histology and Pathology E. CARBOHYDRATE & MUCOSUBSTANCE STAINS: Crystal Violet Stain for Amyloid Stain results Crystal Violet Stain for Amyloid. Showing all 2 results. Acetic Acid, 1%, Aqueous E-301-2. E-301-2 . Select options; Crystal Violet Solution E-301-1. E-301-1 . Select options; Also Available: Fixing Fluids, Dehydrating Agents, Clearing Agents, Embedding Materials, Mounting Media. Red; typical for staining amyloid fibres Image is cutaneous amyloid: Crystal violet: Violet; can stain glia and neurons : Eosin: Pink/orange/red; typical for general staining when combined with haematoxylin Image is of normal skin: Fontana-Masson: Black/pink or red; stains melanin : Giemsa: Blue/violet/pink; commonly used in blood or bone. A, Amyloid with positive Congo red stain. B, Apple-green birefringence. for several months did not influence the results of Congo red stain in either patients or controls. How- ever, smudged nuclear material, caused by freezing and storing fluid samples, may show metachromatic staining with crystal violet that simulates the stainin

and crystal violet staining. No organisms were identified on the microbiology studies. The studies for amyloid were suggestive of amyloidosis, but ultimately were deemed in- conclusive secondary to an inadequate amount of spe- cimen. An MR examination (GE Signa, 1.5 Tesla) of the cer amyloid deposits were detected around the blood vessels in the bone marrow biopsies. There was a complete correlation of crystal violet and Congo red stains, and all of the 31 specimens were positive for amyloid with both of these stains (Figs. 1 and 2). Immunoperoxidase staining proved that the deposits were AA type amyloid in these 31. The amyloid may be seen with several stains, including methyl violet, crystal violet, thioflavin T and Congo red. Congo red is one of the most common staining techniques, as amyloid shows a characteristic apple green birefringence when viewed under polarized light. An H&E stain may give suspicion for amyloid diagnosis, but Congo

Other special stains that can be used to detect amyloid deposits include crystal violet, methyl violet, periodic acid Schiff (PAS), Sirius red, pagoda red, Dylon stain, and Thioflavin T ( Fig. 47.3B ) . AA (but not AL) amyloid loses its affinity for Congo red after exposure to potassium permanganate ings include pink amorphous deposits representing amyloid in the papillary dermis. The overlying epidermis is typically hyper-keratotic and acanthotic. Positive staining with congo red and crystal violet stains can assist with the diagnosis.2,4 n The authors have no relevant conflicts of interest. Allison Cruse, MD is PGY3, Department of. Abstract. The sodium sulphate-Alcian Blue (SAB) method, which stains amyloid green, was evaluated in 220 hearts from elderly patients. The technique proved superior to the Congo red, crystal violet, and thioflavine T methods used either singly or as a battery for the demonstration of cardiac amyloid

Histopathological examination of a punch biopsy specimen showed deposition of homogeneous, eosinophilic material in the papillary dermis. This amorphous, eosinophilic material was stained metachromatically with crystal violet stain and found to be compatible with amyloid sections stained with crystal violet (fig 4). In two cases there were well-marked amyloid deposits which had failed to stain satisfactorily with crystal violet; these were satisfactorily stained with the SABmethod. Withminordeposits the SAB-stained sections showed a more extensive fine network of amyloidfibres thanwasapparent withthioflavine T. Abstract. Congo red (CR) is the most popular dye used as a probe for diagnosing amyloidosis, a very heterogeneous group of diseases with more than 23 chemically different amyloid syndromes of men and animals, leading to more than 400 different individual diseases The crystal violet stain demonstrated the purple colored amyloid material. Dyschromic amyloidosis 152 Figure 1. Diffuse hyperpigmentation with numerous spotted and hypopigmented macules that was located on the first patient's trunk. Figure 3. Deposition of the amyloid in the papillary dermis with histochemical examination of crystal violet.

since amyloid A loses its affinity to Congo red stain and its typical polarizing characteristics af- ter exposure to potassium permanganate, whereas amyloid L still possesses a potassium- permanganate-resistant nature. Crystal violet stain was routinely employed in the detection of amyloid deposits in our series Crystal/Methyl violet for amyloid • Crystal violet or methyl violet stain are used for metachromatic amyloid staining. • They stain amyloid as purple-red in blue background. Reagents used • Crystal/methyl violet • 95% alcohol • 1% aqueous ammonium oxalate • 0.2% acetic acid 70 crystal violet stain, amyloid (red) in heart crystal violet stain, rectal positive for amyloid (red) Despite its amorphous appearance on light microscopy, electron microscopy shows that 90% of any type of amyloid consists of non-branching fibrils, 70 to 100 angstroms (7-10 nm) across. These are usually crisscross but may be parallel Pathology - MCQ 40 - Amyloid stain. Amyloid deposits stain positively with all of the following except: a) Congo-red. b) Crystal violet. c) Methenamine silver. d) Thioflavin T. Correct answer : c) Methenamine silver (Factual question SURGICAL PATHOLOGY - HISTOLOGY Date: STAINING MANUAL - CARBOHYDRATES Page: 1 of 3 CONGO RED - PUTCHLER'S MODIFICATION - AMYLOID PURPOSE: To demonstrate amyloid deposits in tissue sections. PRINCIPLE: Amyloid is homogeneous and eosinophilic, the deposits are extracellular and may become sufficiently large enough to cause damag

Crystal Violet stain for Amyloid - Allina Health Laborator

(B) Intracellular deposits of amyloid, easily identified with crystal violet stain on frozen sections. ( C ) Scattered cytochrome-oxidase-negative fibers, indicative of abnormal mitochondrial. Crystal Violet Amyloid Reticulum Reticulum Fibers Elastic Stain (EVG) Elastic Fibers, Collagen Sodium Sulphate-Alcian Blue (SAB) Amyloid Fontana-Masson Argentaffin Granules or Melanin Thioflavin S Amyloid Giemsa (Mast Cell) Eosinophilic Granules and Mast Cells Toluidine Blue Mast Cell Granules Iron Stain Iron Uric Acid Urate Crystals Jones.

Methods for staining amyloid in tissues: a revie

However, we confirmed the diagnosis by crystal violet staining. Our patient showed positive IF findings for IgM, IgA, and C3 in the perivascular areas. IF techniques are more reliable for detecting lambda or kappa LC in AL amyloidosis than are IHC techniques. However, we were unable to perform IF techniques in our patient The sodium sulphate-Alcian Blue (SAB) method, which stains amyloid green, was evaluated in 220 hearts from elderly patients. The technique proved superior to the Congo red, crystal violet, and thioflavine T methods used either singly or as a battery for the demonstration of cardiac amyloid In an effort to provide you advantage and enlarge our business enterprise, we even have inspectors in QC Staff and assure you our greatest provider and item for methyl violet stain for amyloid, n n diethylaniline msds, 2-Trimethylbenzenamine, sodium hypochlorite, Our tenet is Reasonable prices, efficient production time and best service We hope to cooperate with more customers for mutual.

with haematoxylin & eosin stain (Figure 2). The crystal violet stain demonstrated purple colored amyloid material (Figure 3). All other investigations (full blood count, biochemical profile, Figure 1. Diffuse hyperpigmentation with numerous spotted and hypopigmented macules that was located on the first patient's trunk. Figure 2 1 through 4). The crystal violet stain demonstrated metachromasia typical of amyloid deposits; congophilia typical of amyloid deposits was demonstrated with the Congo red stain, which also demon­ strated green birefringence with polarization, typical of amyloid deposits. CASE2 A 55-year-old woman was referred for evaluation of an eight Subsequent discoveries have shown amyloid to be a heterogeneous substance that can be derived from a variety of proteins, all of which assume a fibrillar configuration containing β-pleated sheets. Amyloid globules can usually be visualized by light microscopy but are enhanced by crystal violet, thioflavin S, or Congo red stains

Lichen Amyloidosis - Practical Dermatolog

An 81-year-old woman presented with a two-year history of ankle edema that had progressed to generalized edema, ascites, and bilateral pleural effusions over the past one year. A Cope needle biopsy of the pleura demonstrated amyloid deposition by Congo red stain. The crystal violet stain failed to reveal any abnormality Additional stains can be used for confirmation; methyl violet or crystal violet will stain amyloid metachromatically, and thioflavin T will confer amyloid with a yellow-green birefringence under. Stains amyloid in frozen sections and platelet 1 blood Crystal violet 4 Used for counter staining of epithelial sections. Aniline Blue 5 Used for staining blood to differentiate leukocytes Giemsa stain 6 Used as contrast stain for staining Ascaris eggs and RBC and as a bacterial spore stain. Use both as a decolorizer and as a counter stain staining characteristics as described by Jones and Zimmerman.2 These sections were then stained with those preparations conventionally used for the identification of amyloid, ie, crystal violet, Congo red, and thioflavine-T. The meth¬ ods used have been described elsewhere.7·8 Results Our results are summarized in the Tabl

Crystal violet stain (magnification x 650). R. I’arallel section reacted with anti-AA antiserum depicting fluorescence of amyloid deposils (arrows) BRIEF REPORTS Figure 3. A, Frozen section showing rnetachromasia of deposits in the wall of an arteriole (arrows). Crystal violet stain (magnification x 1,700) Liver: Most of the liver is replaced by homogenous, hyaline pink-staining amyloid which has been laid down between the sinusoids and the liver cells. This would stain with Congo red, or would be metachromatic if stained with crystal violet. The liver cords are replaced or compressed, and appear as widely separated thin cords of cuboidal cells Gram stain or Gram staining, also called Gram's method, is a method of staining used to distinguish and classify bacterial species into two large groups: gram-positive bacteria and gram-negative bacteria.The name comes from the Danish bacteriologist Hans Christian Gram, who developed the technique.. Gram staining differentiates bacteria by the chemical and physical properties of their cell walls Amyloid tumors are rare masses that may occur in the heart, generally the atria. A conclusive diagnosis rests on identifying the characteristic apple-green dichroism with the Congo red stain. Amyloid is metachromatic with crystal violet

Leder's Stain Method for Enzyme Activity. Lieb's Method For Amyloid (Crystal Violet) Item number: k029. View Details. Select options. Lieb's Method For Amyloid (Crystal Violet) 1. A tissue containing amyloid: Congo red: 1. Harris Heme 2. wash 3. Alkaline salt 4. congo red 5. dehydrate 6. clear: Congo red results: Amyloid: deep pink to red Elastic tissue: pale pink muclei: blue: Crystal violet: Demonstration of amyloid: Crystal violet: 10% NBF: Crystal violet: 10-12 micrometers: Crystal violet control: Tissue containing.

Pathology Outlines - Amyloi

Best differentiator Mayer's carmalum solution Mordanted dye acting as basic dye and staining acidic substances Celestine blue Recommended for routine staining, good nuclear definition Congo red Best known as indicator May be used in staining axis cylinders in embryos Crystal violet Nuclear or chromatin stain used for staining amyloid in frozen sections and platelets in blood Gentian violet. THE CORRECT ANSWER IS D. Thioflavin T is a fluorescent dye that, in addition to staining amyloid, will stain paneth cells, keratin, zymogen granules and juxtaglomerular apparatus. When staining for highly sulfated acid mucosubstances with Spicer's aldehyde fuchsin - alcian blue method, the pH of the alcian blue solution should be amyloid [am´ĭ-loid] 1. resembling starch; characterized by starchlike staining properties. 2. the pathologic extracellular proteinaceous substance deposited in amyloidosis; it is a waxy eosinophilic material. Amyloid deposits are composed primarily of straight, nonbranching fibrils arranged either in bundles or in a feltlike meshwork; each fibril is. Amyloid stain: A. Congo red B. trichrome stain C. alcian blue D. Sudan IV A. Victoria Blue C. Crystal Violet B. New Fuchsin D. ponceau S from physical injury 2. protect the section from bleaching 3. prevent damage and distortion 4. facilitate staining A. only 1,2 and 3 are correct D. only 2,3 and 4 are correct B. only 1,3 and 4 are. Manufacture and distribution of animal blood products, plasma, serum, microbiological diagnostic kits and quality control products, Buckinghamshire, U

Carbohydrates & Amyloid Flashcards Quizle

amyloid when stained with crystal violet, thioflavine T, and PAS, but did not stain or elicit green birefringence with Congo red. Selective staining of amyloid by Congo red appears dependent upon the fibrillar structure of amyloid and the orderly parallel arrangement the mole- cules of Congo red assume when bound lengthwise to the amyloid Crystal Violet for Amyloid Amyloid Amyloid, mucin, renal hyaline - Red Purple, Background - Blue Field's Stain malaria and HP Bacteria & malaria Helicobacter - Blue, malaria - Red Gram bacteria Gram positive bacteria - Blue, Gram negative bacteria - Red STAINS & DYES Alizarin Red S Calcium Calcium - dark Orange, everything else dar Crystal Violet Amyloid Elastic stain (EVG) Elastic fibers, Collagen Fontana-Masson Argentaffin granules or Melanin Giemsa (mast cell) Eosinphilic granules and Mast cells Iron stain Iron Jones methenamine silver Basement membranes, Reticular fibers Luxol fast blue Myelin Masson trichrome Collagen. Thioflavin (T or S) stain can also be used. Other histochemical stains (e.g., crystal violet, etc) are not only less sensitive, but also less specific. Amyloid typing in renal pathology is unlike other areas of surgical pathology, largely owing to the availability of frozen sections Of Amyloid When Met The Crystal Violet Stain for Staining Immunochemistry Staining .p.214 215 -p. 216 219 . Bridge' is three-step The and The third layer by is a PAP' In this the and are un- third is immune complex of The Layer is ed in acts the the PAP (Fig. 3) In this two can be in the Same by raise

Stainsfile - Metachromasia of amyloi

Crystal violet o For staining amyloid in frozen sections and platelets in blood; Gentian violet o Formed by the mixture of: **CV** **Methyl violet** **Dexterin** Congo red o Stain for axis cylinders in embryos o Used as 4 % aq soln in Kraijan's mtd (elastic tissues, amyloid and myelin) Iodine o Oldest stain o For materialwas shownto beamyloidwiththe use ofspecialstains. Itstainedpinkto red with the Congo red stain, and birefrin¬ genceanddichroismwere seen whenthese sections were examined under light. In addition, crystal violet amyloid stain colored the amyloid a deepviolet. CLASSIFICATION OF AMYLOIDOSIS The classification of amyloidosis is as follows. There may be amyloid infiltration of blood-vessel walls, around individual fat cells, and in pilo-sebaceous units. The clinical diagnosis was confirmed by tongue biopsy on H & E stain, the specimen revealed homogenous eosinophilic aggregates and infiltration with lymphocytes and metachromatic stain with crystal violet Methyl violet 2B is a cationic dye with a triphenylmethane structure. It acts as a selective dye and facilitates the differentiation of Klebsiella pneumoniae from Enterobacter aerogenes and other gram-negative organisms. Methyl violet 2B is harmful if inhaled, swallowed or absorbed through skin, contact may cause damage to skin and eyes The following stains are used to stain amyloid: a. crystal violet b. thioflavin T c. Verhoeff von Gieson d. von Kossa e. Perl's stain 4. The following is true about orbital neuroblastoma: a. it is the result of metastasis b. bilaterality is rare c. it is most commonly found at the zygoma.

Special Stains at DelTech Community College - StudyBlue

Amyloid Stains orange; Crystal violet stain. Lattice corneal dystrophy; Amyloid Stains red/purple; Alcian blue stain. Macular corneal dystrophy; Mucopolysaccharides (e.g. goblet cells, mucus) Stain blue; Colloidal iron stain. Macular corneal dystrophy; Mucopolysaccharide (e.g. goblet cells, mucus) Stains blue; Oil red O stain. Sebaceous cell. amyloid) in molluscum lesions by utilizing periodic acid Schiff (PAS) and Congo red (CR) stains [2]. The amyloid was further confirmed with crystal violet (CV) staining. The MC lesions from ten. Moreover, it showed purple amorphous fissured masses in dermis with Crystal Violet staining (Fig. 3), which prompted the deposition of amyloid. Besides, histopathologic examination of the gastric mucosal revealed the extensive deposition of amyloid in the gland stomach tissues and vessel wall

Video: Primary cutaneous amyloidosis pathology DermNet N

crystal violet stain . The incredibly gentian violet, diamino stilbene disulfonic acid, c10h9no4s, sodium nitrite powder, dimethyl p toluidine, methyl violet stain for amyloid, C10H9NO7S2, 2 naphthol polarity, gentian violet ingredients, sodium nitrate uses Although amyloid may be present in the stroma of MCC, it is often absent in aspirated specimens. When present, extracellular amyloid is positive with Congo red and crystal violet stains. Glial fibrillary acidic protein (GFAP) is an intermediate filament protein found in glial cells, such as astrocytes and ependymal cells Amyloid is eosinophilic (pink) with H&E; weakly PAS-positive (this fact assists in differentiating between amyloid and type IV collagen, and between amyloid and immune deposits, intensely positive); negative with methenamine-silver; it is metachromátic: purple with the violet crystal (unfortunately, relatively large amounts of amyloid must be. Other methods of detection of amyloid include fluorescent stains, e.g., thioflavin T or S, and metachromatic stains such as crystal violet. Immunofluorescence and immunoperoxidase methods are used to identify and classify amyloid proteins in tissues. Antibodies against the P component, proteins AA and AL and FAP have been used with great precision Crystal violet staining solution, for Amyloid. Catalog Number: (100504-578) Supplier: Electron Microscopy Sciences. Description: Crystal Violet Stain for Amyloid, Stain results - Amyloid: Violet-Red, Tissue: Blue. View Product Page. Retrieving Each-+ Crystal.

AMYLOID ANIMAL Control Histology Slides - Newcomer Supply

Study Amyloid and Neuro Stains (Wk 12) flashcards from E E's class online, or in Brainscape' s iPhone Crystal violet PAS Amyloid has high cabohydrate - these are carbohydrate stains 13 Congo Red bonding mechanism and pH Non-ionic Likely hydrogen or other weak bond The amyloid can be highlighted with Congo red staining, crystal violet and methyl violet, and cotton pagoda red dye. • The histologic appearance of ichthyosis vulgaris is often subtle, showing thickened compact orthokeratosis and an attenuated granular layer By light microscopy, amyloid appears amorphous, eosinophilic, and hyaline. Characteristic staining qualities distinguish it from other glassy, pink substances. The Congo-red stain results in a brick-red staining reaction and apple-green birefringence, and amyloid stains metachromatically with crystal violet and methyl violet stains5 Leder's Stain Method for Enzyme Activity. Lieb's Method For Amyloid (Crystal Violet) Item number: mw016 Lieb's Method For Amyloid (Crystal Violet) Item number: k029. View Details. Select options. Lieb's Method For Amyloid (Crystal Violet) Lillie's Method for Turnbull's Blue Reaction. Item number: k081. View Details. Select. Suitability: Certified for use with the Gram stain for bacteria, Flemming triple stain with iodine for chromosomes, Lieb′s amyloid stain and Newton′s crystal violet-iodine technique for chromatin and nucleoli, also used as an acid-base indicator, alcohol denaturant, biological stain, textile dye, and an indicator for copper salts

Crystal violet and Methyl violet are mixtures of basic dye, amyloid selectively reacts with one of the dye components : Crystal violet: the addition of acid to the stain solutions prevents overstaining of the cytoplasmic components: Crystal violet: Fixative - 10% NBF or Alcohol: Crystal violet: 10 to 12 microns : Crystal violet: control. scrapings stained with crystal violet. The clumps and streaming both stain purple and both represent biofilms. The amyloid that forms the infrastructure of biofilms is what is being stained. Citation: Allen HB, Goyal K, Ogrich L, Joshi S (2015) Biofilm Formation by Malassezia Furfur/Ovale as a Possible Mechanism of Pathogenesis i

Cureus | Comparison of Immunostaining with HematoxylinPtosis and Dermatochalasis as Presenting Signs in a Case

Because colloid cannot be distinguished from amyloid under light microscopy alone and because colloid, like amyloid, stains positively for periodic acid-Schiff stain, it can be difficult to distinguish it from amyloid. However, colloid is usually negative for the amyloid stain methyl (crystal) violet Special Stains. Special stains are used to differentiate specific components seen in the H&E-stained section. As an example, most pigments can be seen in the H&E-stained section but they all stain basically the same. To determine the makeup of the pigment, special stains are used to differentiate , for instance, copper from iron pigment The darkest of the methyl violet dyes (Gentian violet; methyl violet 10B), used in Gram staining, for the metachromatic staining of amyloid, and as an enhancer for bloody fingerprints. Also a pH indicator, turning yellow below pH 1.8. Its use to treat skin infections is inadvisable since it is probably carcinogenic Amyloid deposits stain with congo red, PAS, Masson's trichrome, thioflavine-T fluorochrome, and are metachromatic with crystal violet. As with all amyloid, they demonstrate apple-green birefringence under polarized light. On electron microscopy the deposits appear as electron-dense, randomly aligned fibrils, 8-10nm in diameter Histological Staining - Carbohydrate and Mucoproteins : Congo Red for Amyloid [Class II] Crystal Violet for Amyloids [Class II] Periodic AcidSchiff's [PAS] [Class I] Mayer's Mucicarmine Method for Mucin and Cryptococcus [Class II] Alcian Blue for Mucosubstances [Class I] Histological Staining - Connective Tissue and Muscl cosal vessels, more typical of gastrointestinal amyloid deposition. Submucosal vessels appeared histologically unremarkable. Special stains for amyloid including Congo red and crystal violet lacked congophilia, apple-green birefringence or metachromasia. Elastic staining (EVG) was positive for thick, truncated and randomly dis